Publications: Peer-reviewed journal articles (by staff)

Detection and identification of furan fatty acids from fish lipids by high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry

  • Uchida H, Itabashi Y, Watanabe R, Matsushima R, Oikawa H, Suzuki T, Hosokawa M, Tsutsumi N, Ura K,
  • Donato Romanazzi,
  • and Miller MR
5 January, 2018
CITATION

Uchida H, Itabashi Y, Watanabe R, Matsushima R, Oikawa H, Suzuki T, Hosokawa M, Tsutsumi N, Ura K, Romanazzi D, Miller MR 2018. Detection and identification of furan fatty acids from fish lipids by high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry. Food Chemistry, in press.

DOI link here

ABSTRACT

Using high-performance liquid chromatography coupled to high-resolution electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOF-MS), we have developed a new method for detection and identification of furan fatty acids (F-acids), which are widely distributed in living organisms and foods as minor lipid components and are known to have antioxidant and anti-inflammatory effects. For this purpose, total fatty acids prepared from the testis lipids of Japanese chum salmon (Oncorhynchus keta) were examined without any concentration or isolation of F-acids. In negative ESI mode, F-acids gave a prominent [M – H]- ion, by which individual F-acids could be detected and identified. High-resolution extracted ion chromatograms clearly showed the occurrence of five major F-acid homologs as already reported by GC/MS. The method was successfully applied to several fish samples and revealed the occurrence of F-acids for the first time in the two New Zealand fish, hoki (Macruronus novaezelandiae) and school shark (Galeorhinus galeus).